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Kev txiav txim txog ntawm Gingerols thiab Shogaols hauv qhav P. E. ntawm HPLC

Source:Apex | Updated: Mar 01, 2016

Kev txiav txim txog ntawm Gingerols thiab Shogaols hauv qhav P. E. ntawm HPLC

Tau:Extract qhiav cagmuaj gingerols thiab shogaols, uas yog ib tug complex sib tov sib xyaw nrog rau 6-gingerdiol, 6-Gingerol, 8-gingerdiol, 8-Gingerol, 6-Shogaol, 10-Gingerol, 8 shogaol, thiab 10-shogaol thiab lwm yam. Hom no yuav muab siv thiab yamntxwv ntawm Gingerols thiab Shogaols rau cov hmoov ntawd qhiav extract.

Tej yam mob I.HPLC:

Qhia kiag: Inertsil ODS-3 4.6 × hli 150 5μm
Txaus tus nqi: 1.0 mL/min
Kom paub tias: 282nm
Volume txhaj: 10μl
Theem txawb: A: Acetonitrile B: 0.1% phosphoric acid dej tshuaj

T(min)

A(%)

B(%)

0

45

55

8

50

50

15

55

45

40

90

10

45

55

45

55

55

45

II. kev hais haum rau hauv lub cev

1. txheem noj:
Yog uas 5mg (±0.01 mg) ntawm capsaicin siv txheem rau ib 50-mL volumetric hwj, yaj thiab dilute los ntim rau methanol.

2. coj noj:
Yog li ntawm 100mg ntawm Ginger Extract rau ib 50ml volumetric hwj luj. Ntxiv li 30 ml methanol, sonicate rau 30mins. Tom qab txias los sov, nta ntim rau methanol. Lim los ntawm ib tug 0.45μm lim ua tus HPLC vial los yog centrifuge kom tau ib cov kua ntshiab mus kuaj.

III. yog muab xam

Yamntxwv txhua compound tiv thaiv capsaicin li lwm txuj.

TypicalchromatogramoftheGingerRootExtract

* Cia lub sij hawm:(reference value)

Compound

6-gingerdiol

6-gingerol

8-gingerol

6-shogaol

10-gingerol

8-shogaol

10-shogaol

RelativeRetentionTime

0.83-0.86

1.00

1.28-1.38

1.38-1.49

1.67-1.82

1.83-2.02

2.13-2.20

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