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Kev txiav txim ntawm Gingerols thiab Shogaols hauv qhiav PE los ntawm HPLC

Source: | Updated: Jan 24, 2018

I. HPLC Li Cas:

 

Kem: Inertsil ODS-3 4.6 × 150mm 5μm

Txaus tus nqi: 1.0mL / min

Tshawb nrhiav: 282nm

Txhaj Cawv: 10μl

Txawb theem: A: Acetonitrile   B: 0.1% kua dej phosphoric acid

T (min)

A (%)

B (%)

0

45

55

8

50

50

15

55

45

40

90

10

45

55

45

55

55

45

 

II. Tshuaj Npaj

 

1. Kev Npaj Txhij:

Tus ccurately weighing 5mg (± 0.01mg) ntawm tus qauv siv capsaicin mus rau ib lub hwj huam 50-mL volumetric , yaj thiab dilute kom ntim nrog methanol.

 

2. Kev Npaj Ua Qauv:

Tshaj li ntawm 100mg ntawm Ginger Extract mus rau ib lub hwj huam 50ml volumetric. Ntxiv txog 30 ml methanol, sonicate rau 30mins. Tom qab txias rau chav sov, dilute kom ntim nrog methanol. Lim ntawm 0.45μm lim rau hauv HPLC vial lossis centrifuge kom tau ib qho kev ntsuam xyuas meej.

 

III. Muab xam

 

Txiav txhua qhov compound tiv thaiv capsaicin li txheej txheem sab nraud.

 

* Cov Sij Hawm Rov Qab: ( tus nqi siv )  

Compound

6-gingerdiol

6-gingerol

8-gingerol

6-shogaol

10-gingerol

8-shogaol

10-shogaol

Txheeb Ze Txeeb Qub

 

0.83 - 0.86

 

1.00

 

1.28 - 1.38

 

1.38 - 1.49

 

1.67 - 1.82

 

1.83 - 2.02

 

2.13 - 2.20

 


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