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Qhov txawv ntawm HPLC, UV yog dab tsi

Source:wikipedia      Date:Dec 22, 2015

HPLC

Kev ua kua chromatography

Kev ua kua chromatography (HPLC; thaum ub hu ua hu ua tshuab muaj muaj zog ua kua chromatography), yog ib tug txheej txheem hauv analytical txhob siv cais, paub, thiab yamntxwv rau ib qho twg rau hauv ib cov dej. Nws nyob twj kom dhau ib pa ua kua hnyav uas muaj tus qauv dej ntawm ib sab muaj ib adsorbent khoom khoom relies. Ib qho twg ntawm cov pawg laag txawv me ntsis uas cov adsorbent, yuav ua tau rau ntau txaus rau cov nqi ntawm cheebtsam ntau thiab ua rau qhov kev sib cais ntawm lub Cheebtsam thaum lawv ntws tawm sab.

HPLC twb tau muab siv kom raug (e.g. thaum ntau lawm txoj kws thiab lom khoom), raws li kev cai (xws li tebchaws kev ua tau zoo li tshuaj hauv zis), tshawb xyuas (xws li nkauj nraug lub Cheebtsam ntawm txoj me coj, los yog tshuaj zoo xws li cov khoom cua sib), thiab hais txog kev kho mob (xws li tebchaws vitamin d kom ntau ntxiv nyob rau hauv cov ntshav)

Feem ntau lub sij hawm, HPLC yuav siv tau los qhia herbal extract.

UV

Ultraviolet

UV/VIS spectroscopy yog lug ib txheej txheem nyob txhob siv mus txheeb xyuas cov qauv tshuaj, yog tus notable tshaj ib rhais conjugated lub nruab. UV tawg ntau kuj yuav siv los ua kom muaj siab tus qauv zauv uas cov qhov fluorescent emission yog ntsuas rau ib spectrofluorometer. Hauv kev soj ntsuam me, sib UV yog siv quantification nucleic kua qaub los yog cov nqaijrog.


Ib phau ntawm ntxhia kuaj brilliantly fluorescing ntawm cov wavelengths ntau raws li pom thaum irradiated los ntawm sib UV.

Ultraviolet roj kuj siv hauv cais cov zaub mov thiab gems.

Hauv paug tswj kev siv, ultraviolet analyzers yuav siv los xyuas txog cov nitrogen oxides emissions, leej faj tebchaw, mercury thiab ammonia, xws li hauv lub nkev flue cov pob txha raug rho tawm haujlwm fais fab tuaj cov zaub. [48] ultraviolet lub teeb yuav ntes nyias sheens ntawm cov roj spilled hauv dej, xws li tus siab reflectivity ntawm cov roj films nyob UV wavelengths, cov tebchaw rau roj, fluorescence los yog absorbing ntawm lub teeb UV uas Raman scattering hauv dej.

Nyob rau hauv cov khoom xyaw herbal teb, peb siv no txoj kev sim feem pua ntawm yam muaj nyob hauv tsob nroj extract.

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